Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (29): 5336-5340.doi: 10.3969/j.issn. 2095-4344.2012.29. 004

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Ordered nanopore arrays on the surface of diameter-controlled anodic alumina influence the adhesion behavior of umbilical vein endothelial cells

Ni Si-yu1, Zhang Yan-fei1, Ni Shi-rong2   

  1. 1College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China;
    2Department of Pathophysiology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China
  • Received:2011-11-15 Revised:2011-12-16 Online:2012-07-15 Published:2012-07-15
  • About author:Ni Si-yu☆, Doctor, Associate researcher, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China synicn@dhu.edu.cn

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Abstract:

BACKGROUND: Studies have shown that many types of cells can influence the surface structure of micrometer scale on material surfaces.
OBJECTIVE: To observe the effect of various pore sizes of ordered nanopore arrays on diameter-controlled anodic alumina on the adhesion behavior of human umbilical vein endothelial cells (hUVECs).
METHODS: The order arranged porous anodic alumina (PAA) with controllable pore sizes (25, 50, 65 or 75 nm) was prepared using a two-step anodizing procedure in oxalic acid electrolytes. The hUVECs were seeded on the PAA surface with different pore sizes to evaluate the cellular adhesion behavior on the PAA surface.
RESULTS AND CONCLUSION: The hUVECs adhered well on the surfaces of all the PAA specimens and showed a normal morphology. MTT assay showed that the hUVECs adhesion rates were significantly higher on the surface of the PAA with larger diameter of 75 nm compared with the PAA with smaller diameter of 25 nm after 4 and 24 hours of culture (P < 0.05). It is indicated that the PAA with larger diameter of 75 nm can promote the cell adhesion compared with the PAA with smaller diameter of 25 nm based on its better biological activity.

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